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The High Content Screening (HCS) Facility supports image-based high content assays utilizing high throughput microscopy. The HCS team has expertise in all areas of high content image-based screens including sample preparation, image acquisition, image analysis, image data management, and algorithm development. The HCS facility also supports the screening efforts of the Functional Genomics Shared Resources, providing expertise for HCS assays using RNAi libraries.
Assay Conceptualization:
Single-Plate Experiments & Small-Scale HCS (Plate Preparation by PI Lab Scientest):
Medium- and Large-Scale Screens (Plate Preparation by HCS or Functional Genomics Facility):
Dara Analysis and Image-data Management:
Assay Guidance Manual: http://www.ncbi.nlm.nih.gov/books/NBK100913/
Nikon Microscopy: http://www.microscopyu.com
Zeiss Microscopy: http://zeiss-campus.magnet.fsu.edu/index.html
Olympus Microscopy: http://www.olympusmicro.com/primer/anatomy/anatomy.html
Recommended 96-well plates: | |
PerkinElmer CellCarrier-96 Ultra Microplates (preferred) | Cat. # 6055302 |
Greiner 96-well culture Microplate | Cat. # 655090 |
Matrical (glass-bottom) | Cat. #MGB096-1-2-HG |
Recommended 384-well plates: | |
PerkinElmer CellCarrier-384 Ultra (preferred) | Cat. #6057302 |
Greiner 384-well microplates | Cat. #781091 |
Aurora 384-well ultra low base | Cat. # 00032451 |
Matrical (glass-bottom) | Cat. #MGB101-1-2-HG |
Recommended 1536-well plates: | |
Aurora Low base black clear microplates (preferred) | Cat. # 00029726 |
PerkinElmer CellCarrier 1536-well microplates | Cat. # 6004550 |
Recommended 3D Spheroid plates: |
Nexcelom U-bottom (preferred for Celigo only) | https://shop.nexcelom.com/collections/plates |
For all other plates, please contact us before starting your experiment! |
Information on general plate selection for HCA / HCS can be found here: https://www.perkinelmer.com/lab-products-and-services/application-support-knowledgebase/microplates/high-content-screening-plates.html
Susanne Heynen-Genel | Director
Hours | Location |
24/7 Appointment Only |
Instrument Access: La Jolla, Bldg 2 - Room 2201 HCS Staff for Consulting, Training, Assistance |
Name | Role | Phone | Location | |
---|---|---|---|---|
Susanne Heynen-Genel |
Facility Director
|
858-646-3100 x3329
|
sheynen@sbpdiscovery.org
|
Room 2222
|