ALERT

In observance of the upcoming holidays, Agilent Cross Lab iLab Operations Software will be closed during US hours on Monday December 25th, 2017 and Monday January 1st, 2018. EU and APAC will be closed on Tuesday December 26th, 2017. During the week, we will have limited support hours and resume regular support hours on Tuesday January 2nd, 2018. For urgent matters, please add "URGENT" to the ticket subject and these requests will be prioritized first.

 

 

To submit a help ticket with iLab, click on the HELP link to access the support portal. If you are unable to access the support portal, email ilab-support@agilent.com and this will create a ticket. iLab Support can also provide you with access to the support portal.

High Content Screening Facility (La Jolla)

Getting Started

Internal SBPMDI

  • If you have an iLab account, please login at the top right or use this link https://ilab.sbpdiscovery.org/
  • If you do not have an iLab account, please click HERE to request an account. All fields are required.

 

External SBPMDI

  • You must have a Customer ID and iLab account to request services from this facility.
  • To obtain a Customer ID please email Eveline Hernandez at ehernandez@sanfordburnham.org
  • To obtain an iLab account, please click HERE.


Forgot your password? Please click HERE.

 

 

Overview of Services

The High Content Screening (HCS) Facility supports image-based high content assays utilizing high throughput microscopy. The HCS team has expertise in all areas of high content image-based screens including sample preparation, image acquisition, image analysis, image data management, and algorithm development. The HCS facility also supports the screening efforts of the Functional Genomics Shared Resources, providing expertise for HCS assays using RNAi libraries. 

 

Assay Conceptualization:

  • Initial consultation for biological feasibility
  • Aid in selection of reagents, cell lines, and assay conditions

 

Single-Plate Experiments & Small-Scale HCS (Plate Preparation by PI Lab Scientest):

  • Use of HCS instruments including training
  • Use of analysis software including training
  • Develop and validate image analysis algorithms and assay read-outs

 

Medium- and Large-Scale Screens (Plate Preparation by HCS or Functional Genomics Facility):

  • Optimize assay biology
  • Develop image analysis algorithms and assay read-outs
  • Perform assay validation, miniaturization, and implementation
  • Screen in collaboration with HTS or Functional Genomics facilities

 

Dara Analysis and Image-data Management:

  • Perform normalization, hit selection, and dose response
  • Storage of images and raw data
  • Aid with images and data visualization

 

High Content Assays - Useful Links

Assay Guidance Manual: http://www.ncbi.nlm.nih.gov/books/NBK100913/

Nikon Microscopy: http://www.microscopyu.com

Zeiss Microscopy: http://zeiss-campus.magnet.fsu.edu/index.html

Olympus Microscopy: http://www.olympusmicro.com/primer/anatomy/anatomy.html

 

High Content Plate Recommendations 

Recommended 96-well plates:

 

BD Falcon 96-well black/clear plates (preferred)

Cat. # 353219

Greiner 96-well culture Microplate

Cat. # 655090

Matrical (glass-bottom)

Cat. #MGB096-1-2-HG

Recommended 384-well plates:

 

Perkin Elmer CellCarrier-384 Ultra (preferred)

Cat. #6057300

Greiner 384-well microplates 

Cat. #781091

Aurora 384-well ultra low base  

Cat. # 00032451

Matrical (glass-bottom)

Cat. #MGB101-1-2-HG

Recommended 1536-well plates:

 

Aurora Low base black clear microplates (preferred)

Cat. # 00029726

Greiner 1536-well microplates

not tested yet

Matrical (glass-bottom) 

Cat. #MGB111-1-2

Recommended 3D Spheroid plates (Celigo):

 

Nexcelom U-bottom (preferred)

https://shop.nexcelom.com/collections/plates

 

Leadership

Susanne Heynen-Genel | Director

Location and hours of operation

Hours Location

24/7

La Jolla, Bldg 2 - Room 2201

 

Contacts

Name Role Phone Email Location
Susanne Heynen-Genel
Facility Director
 
858-646-3100 x3329
 
sheynen@sanfordburnham.org
 
Room 2222
 

Services and Prices


Search available services: View: by category alphabetically
Equipment Rates (1)