Functional Genomics (La Jolla)

Getting Started

Internal SBPMDI

• If you have an iLab account, please login at the top right or use this link https://ilab.sbpdiscovery.org/
• If you do not have an iLab account, please click HERE to request an account. All fields are required.

External SBPMDI

• You must have a Customer ID and iLab account to request services from this facility.
• To obtain a Customer ID please email Chun-Teng Huang at cthuang@sbpdiscovery.org
• To obtain an iLab account, please click HERE.

Forgot your password? Please click HERE.

Overview of Services

Functional Genomics provides infrastructure for CRISPR-based gene editing and Target Discovery and Deconvolution services via high throughput screening, from initial feasibility assessment, all the way through to verification of identified targets. It also serves as repository for reagents and assays, expertise and other technical information, and works towards technical improvements when necessary.

The Core’s primary effort is dedicated to cell-based genetic screening, both loss of function (via RNAi, CRISPR-Cas9 screening) and gain of function (via ORF overexpression and CRISPRa screening). In addition it offers cell line engineering services to create cellular models for screening and/or target validation.

Our personnel develop both plate reader and image-based cellular assays, and adapt them to HTS screening conditions in conjunction with the High Content Screening Core. It also collaborates with the Bioinformatics, Proteomics and Sequencing Shared Resources to aid the screening follow-up process.

Services:

Cell-based Genetic Screening:

• Assay development.
• Screening (siRNA, CRISPR-Cas9, lentiviral-shRNA, lentiviral-ORF libraries).
• Custom library production.
• Hit cherry-picking and confirmation.
• Screen readout (high content, plate reader, NGS)

Cell line engineering.

• Gene KO
  
• Endegenous reporters of gene expression
  
• Endogenous gene activation
  
• Endogenous protein tagging
• Gene editing

Leadership

Chun_Teng Huang | Acting Director

Location

Contacts