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The Protein Production Core Facility at Lake Nona is dedicated to function as a full service and shared resource facility providing custom protein production and analysis expertise to Sanford Burnham investigators and throughout the scientific community. With the availability of specialized protein production scale up services including consultation, technical support and optimization of production runs, the core offers state-of-the-art facilities with bioprocessing capabilities that meet the needs of clients alongside the highest quality standards. The Protein Production core facility offers protein expression services based on 3 recombinant platforms consisting of mammalian, microbial and insect based systems. In each case a small scale test expression, expression optimization, and large scale expression package can be provided. The primary expression systems used are E.Coli, suspension based CHO or HEK293, and Sf9 insect cells. With the ability to utilize three platforms the core can produce a broad range of proteins for a variety of downstream applications with operations going from research lab based protein yields to scaled up protein production yields using pilot scale bioreactor/fermenter systems. With the implementation of advanced biochemical engineering techniques protein yields can be significantly increased.
Our analytical services are focused on biophysical characterization of structural and functional properties of proteins using several methods including the latest surface plasmon resonance techniques. We can perform quality control of protein samples and measure protein-protein interactions. The Core can also assist in experimental design using advanced statistical optimization techniques.
Protein Production Core Policy:
Due to the customized nature of protein productions, all protein production service fees are charged in advance and once a production is initiated there will be no refunds. However, subsequent services will be discounted less the amount of the actual labor and expenses on the previous cancelled project.
Cell culture and protein expression can have varying time frames and successes depending on the product required. Thus we can not guarantee soluble protein yield. The Core will explore varying expression strategies with the goal of maximizing soluble protein expression. Purified protein yield depends on expression level and preparation used. The detailed results will be delivered to the customer for every project.
For all training services the service fee is charged in advance. Fees for the instrument usage and sample analysis are charged after the service is provided while fees for the sample analysis are charged strictly on the bases of time involved in conducting the experiment. Sample handling fee will be charged if additional steps are necessary to perform the experiment (protein concentration measurement, buffer exchange, sample concentrating, etc.).
Darrin Kuystermans (Ph.D.) | Associate Director, Protein Production & Analysis Core Facility |
Hours | Location |
8AM to 5PM |
SANFORD-BURNHAM MEDICAL RESEARCH INSTITUTE at LAKE NONA 6400 Sanger Road, Orlando, FL 32827 |
Name | Role | Phone | Location | |
---|---|---|---|---|
Dr. Darrin Kuystermans |
Associate Director
|
407.745.2144
|
dkuystermans@sbpdiscovery.org
|
A2840
|
Prices and Services |
► 1. Cloning Services (2) | |||
Name | Description | Price | |
---|---|---|---|
1. Cloning/Vector Engineering, per construct (Plasmids at 20ug) | Inquire | ||
2. Cloning/ sequencing, each additional 500 bp | Inquire | ||
► 2. Microbial Based Platform Protein Production Services (9) | |||
Name | Description | Price | |
1. E.coli expression trials, per construct | Inquire | ||
2. Protein production from E.coli 1L culture (with one GST or His-tag affinity purification step included) | Inquire | ||
3. Protein production from E.coli, each additional liter up to 24L (for LNPPC202) | Inquire | ||
4. Protein production from E.coli in SIP fermenter, 30L | Inquire | ||
5. Protein production from E.coli in SIP fermenter each additional liter up to 50L | Inquire | ||
6. Cell pellet production, E.coli, per 1 liter (for 12L plus cultures) | Inquire | ||
7. Cell pellet production at 50L scale | Inquire | ||
8. Cell pellet production at 100L scale | Inquire | ||
9. Cell pellet production at 200L scale | Inquire | ||
► 3. Insect Based Platform Protein Production Services (7) | |||
Name | Description | Price | |
1. Baculovirus, virus production, per construct | Inquire | ||
2. Insect expression trials, per construct | Inquire | ||
3. Insect Cell Pellet Production, per liter | Inquire | ||
4. Protein production in insect cells, from 1 liter culture | Inquire | ||
5. Protein production in insect cells in bioreactor, 2 liter | Inquire | ||
6. Protein production in insect cells in bioreactor, each additional liter up to 10L | Inquire | ||
7. Protein production in insect cells in bioreactor, at 25L | Inquire | ||
► 4. Mammalian Based Platform Protein Production Services (5) | |||
Name | Description | Price | |
1. Mammalian expression trials, per construct | Inquire | ||
2. Protein production in mammalian cells, from 400ml culture | Inquire | ||
3. Protein production in mammalian cells in bioreactor, 1 liter | Inquire | ||
4. Protein production in mammalian cells in bioreactor, each additional liter up to 10L | Inquire | ||
5. Protein production in mammalian cells in bioreactor, at 25L | Inquire | ||
► 5. Protein Purification Services (13) | |||
Name | Description | Price | |
01. Pellet/Lysate protein purification (tagged constructs) up to 200ml | Inquire | ||
02. Pellet/Lysate protein purification (tagged constructs) 200ml to 1L | Inquire | ||
03. Pellet/Lysate protein purification (tagged constructs) each additional liter | Inquire | ||
04. Protein purification, optimization, E.coli, per construct | Inquire | ||
05. Protein purification, optimization, insect cells, per construct | Inquire | ||
06. Protein purification, optimization, mammalian cells, per construct | Inquire | ||
07. FPLC purification, per sample with 1x5ml column | Inquire | ||
08. FPLC purification, per sample each additional 1x5ml column | Inquire | ||
09. Extra FPLC SEC purification step after FPLC affinity purification (per 10 ml sample volume for SEC) | Inquire | ||
10. Protein purification (Ni-column or GST and SEC FPLC) from a 100L scale culture | Inquire | ||
11. Protein purification (Ni-column or GST and SEC FPLC) from a 200L scale culture | Inquire | ||
12. Protein purification (Ni-column or GST only) from a 100L scale production culture | Inquire | ||
13. Protein purification (Ni-column or GST only) from a 200L scale production culture | Inquire | ||
► 6. Protein Analysis Services (3) | |||
Name | Description | Price | |
1. ITC experiment, per titration | Inquire | ||
2. ProteonXPR36-SPR experiment, per SPR array used | Inquire | ||
3. Size Exclusion Chromtagraphy for Multi-Angle Light Scattering (SEC-MALS) | Inquire | ||
► 7. Equipment Training and Use (9) | |||
Name | Description | Price | |
1. ITC training | Inquire | ||
2. ITC use, daily |
Daily Rate |
Inquire | |
3. UV/Vis Spectrophotometer training | Inquire | ||
4. ProteonXPR36 -SPR training (includes array costs) | Inquire | ||
5. ProteonXPR36 -SPR use, weekly (array not provided) |
Weekly Charge |
Inquire | |
6. FPLC use, daily without columns | Inquire | ||
BD FACS Caliber Training (per minute) | Inquire | ||
Charge for data analysis on instrument computer (per minute) | Inquire | ||
FACS Caliber Sort Training (per minute) | Inquire | ||
► 8. Extra Services (7) | |||
Name | Description | Price | |
1. Consultation training, data analysis, per minute |
Cost Per Minute |
Inquire | |
2. Supplies | Inquire | ||
3. Outsourced service | Inquire | ||
Falcon Filter Tubes (Each) | Inquire | ||
Falcon Filter Tubes (per Bag) | Inquire | ||
Falcon Tubes w/caps (Each) | Inquire | ||
Falcon Tubes w/caps (per Bag) | Inquire |