Cell Imaging Core (La Jolla)

Getting Started

Internal SBPMDI

• If you have an iLab account, please login at the top right or use this link https://ilab.sbpdiscovery.org/
• If you do not have an iLab account, please click HERE to request an account. All fields are required.

 External SBPMDI

• You must have a Customer ID and iLab account to request services from this facility.
• To obtain a Customer ID please email Leslie Boyd at lboyd@sbpdiscovery.org
• To obtain an iLab account, please click HERE.

Forgot your password? Please click HERE.

Overview of Services

 The core houses a wide variety of microscopes, and provides training, assistance and use of the instruments, as well as full-service imaging.  The Cell Imaging Facility microscopes include  a Nikon N-SIM Super-Resolution/A1 confocal microscope system, seven advanced fluorescence microscopes and three confocal systems (including a  Zeiss  LSM-710 NLO multiphoton microscope, Olympus FluoView-1000 and Yokagawa Spinning Disk confocal).  Sophisticated systems for FRET, Calcium imaging, and TIRF are also available.  The microscopes are integrated with advanced image capture and analysis software, and a number of instruments are equipped with environmental chambers for extended live cell analysis.

Services

• Wide field microscopy:
  • bright field and dark field microscopy
  • phase, polarization, and Nomarski DIC (differential interference contrast)
  • epi-fluorescent microscopy
• Single and multiphoton confocal microscopy
• Super-Resolution microscopy
• Live cell imaging
• Image analysis:
  • morphometry
  • 3D and 4D rendering
  • cell tracking
  • deconvolution
• Training and consultation

 Equipment and Resources

• Nikon N-SIM Super-Resolution/A1 confocal microscope system
• Upright BX50 Olympus wide field fluorescence microscope with cooled, color CCD camera and an automated XYZ stage
• Two inverted TE300 Nikon wide field fluorescence microscopes with cooled, color CCD cameras (the second one with an automated XYZ stage).
• Inverted IX81 Olympus wide field fluorescence microscope for FRET and Ca2+ imaging, equipped with cooled monochrome CCD camera Cascade 512B, XYZ controlled stage and CO2/temperature controlled environmental chamber for long term time lapse imaging
• Inverted IX81 Olympus wide field fluorescence microscope for TIRF time lapse imaging, equipped with cooled CCD monochrome CCD camera QuantEM and Piezo-Z controlled  objective stage; Kr-Ar laser emitting at 488, 568 and 647nm lines
• Inverted IX81 Olympus wide field fully automated fluorescence microscope for basic and advanced methods of microscopy.  Microscope equipped with high resolution cooled monochrome CCD camera ORCA-ER.
• Yokogawa Spinning Disk Laser Confocal Microscope with XY and Piezo-Z controlled objective stage (ASI  2000-Piezo); Kr-Ar laser emitting at 488, 568 and 647 nm lines, cooled, monochrome CCD camera QuantEM; “20/20” environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging
• Zeiss LSM-710 NLO  Multiphoton Laser Point Scanning Confocal Microscope equipped with single photon Ar-ion laser emitting at 457, 479, 488 and 514 nm lines; DPSS solid state laser emitting 561 nm line; Red Diode laser, emitting at 633 nm line, and multi-photon Mai-Tai-HP_ DeepSee Laser; “20/20” environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging.
• Fluoview 1000 Olympus Laser Point Scanning Confocal Microscope, single photon lasers (Diode laser emitting 405nm line; Argon-ion laser emitting at 457, 488 and 514 nm; solid state diod lasers emitting 561 nm and Red Diode laser, emitting at 633 nm); SIM Scanner with 405nm laser diode; Spectral Detection system; FRAP; FRET and photoactivation applications; Tokai Hit environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging
• Software packages for image processing:
  • ImagePro Plus
  • Imaris Single Full Suite with ClearView Deconvolution
  • Volocity 3D-4D rendering
  • Metamorph//MetaFluor
  • Nikon Elements offline
  • Zeiss Zen Black

Leadership

Leslie Boyd | Facility Manager

Location and hours of operation