Flow Cytometry Shared Resource (La Jolla)

Getting Started

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SBP Researchers

• If you have an iLab account, please login at the top right or use this link https://ilab.sbpdiscovery.org/
• If you do not have an iLab account, please click HERE to request an account. All fields are required.

External Researchers from other institutions

• You must have a Customer ID and iLab account to request services from this facility.
• To obtain a Customer ID please email Yoav Altman at yoav@sbpdiscovery.org
• To obtain an iLab account, please click HERE.

Forgot your password? Please click HERE.

Overview of Services

The facility provides access to high-speed cell sorting, analytical flow cytometry and imaging flow cytometry on the Sanford-Burnham campus in La Jolla, CA.  Trained investigators have 24-hr/day access to a variety of analytical flow cytometers available for independent use.  Core staff provide technical expertise, hardware and software training, operate the facility’s cell sorters and are available to assist with analysis experiments for those who prefer to have their samples run by an expert cytometrist.  Scientists planning a flow cytometry experiment are encouraged to consult facility staff for assistance with protocols, fluorochrome selection or other aspects of experiment design.

Services

• High speed cell sorting performed by facility personnel in two locations
• Single-cell (clone) sorting into 96-well or 384-well plates
• Simultaneous sorting of up to 4 populations
• Analytical flow cytometry: do-it-yourself 24hr/day, or assisted by appointment
• Imaging flow cytometry: do-it-yourself or assisted by appointment
• Hardware and software training
• Consultation
• Experiment design
• Data analysis and interpretation
• Pre-publication manuscript review

Equpiment and Resources

• BioRad TC10 Automated Cell Counter

Analyzers

• Amnis ImageStreamX MarkII Imaging Cytometer with: plate loader, 12 imaging channels, 405nm 488nm and 642nm lasers
• Cytek Aurora 5L Full Spectrum Cell Profiler with plate loader
• BD LSRFortessa X20 18-color FACS analyzer with HTS plate loader for 96 or 384-well plates and five solid state lasers (355 nm, 405 nm, 488 nm, 561 nm, 637 nm)
• BD LSRFortessa 14-color FACS analyzer with HTS plate loader for 96 or 384-well plates and four solid state lasers (405 nm, 488 nm, 561 nm, 640 nm)
• ACEA Novocyte 3000 13-color FACS analyzer with 488 nm, 405 nm, 640 nm lasers

 Sorters

• BD FACSAriaIIu 16-color high-speed cell sorter with 405 nm, 488 nm, 561 nm and 640 nm laser lines
• BD FACSAriaIIIu 15-color high speed cell sorter with 407 nm, 488 nm, 561 nm and 633 nm laser lines

 Computer Workstations

• Computer workstations with large monitors and data analysis software packages including
• FlowJo
• ModFit LT
• IDEAS
• SpectroFlo

Price List

Leadership

Yoav Altman | Resource Director

yoav@SBPdiscovery.org

Phone: 858-646-3106

Location and hours of operation

Links and Resources

1. SBP Flow Cytometry Public Webpage
2. SBP Flow Cytometry Intranet Website
   
3. Fluorofinder page

Available Equipment and Resources